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. 2021 Feb 3;13(2):501. doi: 10.3390/nu13020501

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Long-term DHA supplementation and/or aerobic exercise training regulates the expression of key autophagy-related proteins/genes in the liver of 18-month-old DIO mice. (A) Representative Western blot (left panel) and densitometry analysis (right panels) of LC3 I and II and p62. Band densities of p62 were normalized to β-actin. (B) mRNA expression levels of hepatic Atg5 and Atg7. Data are expressed as mean ± SEM. n = 6–10. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. C group. ^# p < 0.05, ^### p < 0.001 vs. DIO group. ^††† p < 0.001 for DIO + DHA vs. DIO + DHA + EX group. ^c p < 0.001 for DIO + EX vs. DIO + DHA + EX group.