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. 2021 Feb 3;22(4):1517. doi: 10.3390/ijms22041517

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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RT-PCR validation of DEGs in the MPOA of pup-deprived and lactating mothers (n = 11 for both groups). (A–C): mRNA levels of genes with higher expression in lactating mothers according to the RNA-Seq. Data, shown as ratio of GAPDH mRNA levels (mean ± SEM). Ndufs5 and Nwd1 mRNA levels were significantly higher in lactating mothers (** = p < 0.01, * = p < 0.05). Expression of Ecm2 did not differ significantly. (D–F): mRNA levels of genes with higher expression in pup-deprived mothers according to the RNA-Seq. Data, shown as ratio of GAPDH mRNA levels (mean ± SEM). Rbm3 mRNA level was significantly higher in pup-deprived mothers (* = p < 0.05). Mepce and Gpr34 expression did not differ significantly.