Figure 7.

Expression and processing of chloroplast rpoC1 transcripts in wild-type (Col-0) and mterf2 mutant plants. (a) RNA-Seq analysis was performed with RNA extracted from wild-type (Col-0) and amiR1-3-mterf2 mutant (amiR) seedlings. The read depths of rps2, rpoC2, rpoC1, and rpoB transcripts detected in Col-0 and amiR seedlings were visualized with the Integrative Genomics Viewer (IGV). Below the IGV data, a schematic representation of the chloroplast rpoC1 gene is shown and the magenta arrows indicate the primer positions used in Figure (b). (b,c) Quantitative RT-PCR analysis of RNAs isolated from 6-day-old Col-0 and amiR1–mterf2 mutant plants (amiR1-1, amiR1-2, and amiR1-3). PCR was conducted with the primer pairs marked in panel (a) and AT4G36800, encoding an RUB1–conjugating enzyme (RCE1) as a control. The RNA expression levels are reported relative to that in the Col-0, which was set to 1. The ratios of spliced to unspliced rpoC1 transcript between amiR1–mterf2 mutant seedlings (amiR1-1, amiR1-2, and amiR1-3) and wild-type (Col-0) were calculated based on the relative levels of rpoC1 exon 1, exon 2, and the intervening intron, and the data are depicted as the log₂ ratio of spliced to unspliced rpoC1 transcript in the amiR1–mterf2 mutant plants compared to Col-0 in (b). Relative RNA levels of exon 1, read through 1 and -2 in Col-0 and amiR1–mterf2 mutant plants are shown in (c). Bars indicate standard deviations (SD). Significant differences between mutants and Col-0 were evaluated with Student’s t-test (P < 0.05) and they are denoted by the asterisks (*).