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. 2021 Feb 3;10(2):112. doi: 10.3390/biology10020112

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The melanization of hemolymph was inhibited by high PPC in silkworm after modeling and could be rescued by exogenous 20E. CK, the control group; AM, animal model of hyperproteinemia in which hyperproteinemia was induced at the wandering stage; AM+20E, the larvae of the AM were rescued by injecting 4 μg of 20E at 24 h after the induction of hyperproteinemia. (A) Melanization speed in vitro of hemolymph. (B) Phenoloxidase (PO) activity in hemolymph. (C,D) The mRNA levels of PPO1 and PPO2 in hemocytes. (E) The mRNA levels of PPO1 in hemocytes at 48 h. (F) PO activity in hemolymph at 48 h after modeling. * p < 0.05; ** p < 0.01; n.s., no significant difference between the two groups. Mean ± SEM. n = 3.