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. 2021 Feb 8;49(4):2065–2084. doi: 10.1093/nar/gkab041

Table 3.

Equilibrium dissociation constants for mutant hRev1330–833 enzyme binding to ss-Myc-14/23 and non-G4 DNA substratesa

K D,DNA Fold preference for G4 DNA
Non-G4 (nM) G4 (nM) (KD,non-G4 DNA/KD,G4 DNA)
L358A 660 ± 140 77 ± 8 9
E466A 1100 ± 130 280 ± 20 4
E466K 1300 ± 300 43 ± 16 30
Y470A 740 ± 180 180 ± 30 5

aThe single-stranded Myc 14/23 G4 and non-G4 DNA substrates were used to measure the binding affinities reported here. Data represent the mean ± SD (n = 3).