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. 2021 Feb 1;49(4):1900–1913. doi: 10.1093/nar/gkab023

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Non-perfectly matching small RNAs can efficiently induce DNA methylation in virus-infected plants. (A) Analysis of DNA methylation at the target CaMV 35S promoter (from -208 to -89) by bisulfite sequencing in TRV-infected N. benthamiana 16c plants. DNA was extracted from the systemically infected leaves at 21 dpi. The histogram shows the percentage of total methylated cytosine. Asterisks indicate significant differences (Student's t-test, P < 0.01). (B) Summary of bisulfite sequencing analysis. Red, blue and green bars indicate the percentage of methylated cytosine residues at CG, CHG and CHH sites, repetitively. Data presented in (A) and (B) were obtained from three independent biological replicates. Raw data are available in Supplementary Figure S6. Error bars represent a confidence interval with 95% confidence limits (Wilson score interval; see details in Supplementary Figure S6B).