Table 1.
Sperm mutagenesis efficiency
| Mutation library | No | PCR positive frequencya | New insertion frequencyb | ET frequencyc | ET transmission frequencyd |
|---|---|---|---|---|---|
| TnE2/Tp62 | 1 | 4/8 = 0.5 | 1/4 = 0.25 | - | - |
| 2 | 3/9 = 0.333 | 2/3 = 0.667 | - | - | |
| 3 | 5/11 = 0.455 | 1/5 = 0.2 | 1/11 = 0.091 | 4/8 = 0.5 | |
| TnE2/Tp191 | 1 | 11/15 = 0.733 | 1/11 = 0.091 | - | - |
| 2 | 3/9 = 0.333 | 0/3 = 0 | - | - | |
| 3 | 10/15 = 0.667 | 1/10 = 0.1 | - | - | |
| TnE2/Tp68 | 1 | 9/13 = 0.692 | 1/9 = 0.111 | - | - |
| 2 | 6/10 = 0.6 | 1/6 = 0.167 | - | - | |
| 3 | 7/13 = 0.538 | 0/7 = 0 | - | ||
| TnE2/Tp64 | 1 | 5/12 = 0.417 | 1/5 = 0.2 | 1/12 = 0.083 | 5/8 = 0.625 |
| 2 | 6/13 = 0.462 | 1/6 = 0.167 | 1/13 = 0.077 | 7/11 = 0.636 | |
| 3 | 3/7 = 0.429 | 1/3 = 0.333 | - | - | |
| Total | 72/135 = 0.533 | 11/72 = 0.153 | 3/135 = 0.022 | - |
aGFP-PCR positive per litter.
bNew insertion of the GFP-PCR positive.
cvisible GFP positive (F1) per litter.
dvisible GFP positive (F2) per ET line litter.
No, number of litters.