Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jan 21;49(4):2027–2043. doi: 10.1093/nar/gkab003

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 3. — Increased apoptosis and activated p53 pathway in RPCs of prpf31⁻^/⁻ zebrafish. (A) TUNEL staining showed numerous apoptotic cells in the retina, brain and posterior segment of spinal cord in prpf31 mutants at 48 hpf. Injection of wild-type prpf31 mRNAs reduced the apoptotic cells to a normal level. Dotted lines indicate the boundary of two images from the same embryo. White arrows, apoptotic signals. Scale bar, 100 μm. (B) The up-regulation of p53 pathway genes in prpf31 mutants at 36 hpf as detected by qPCR. (C) Deletion of p53 in prpf31 mutants significantly reduced cell apoptosis, but could not rescue the differentiation defects of RPCs. Scale bar, 100 μm. (D−F) The quantitative analysis of TUNEL positive cells, Sox2 positive cells and Islet1 positive cells shown in (C). n = 9 for each panel. Scale bar, 100 μm.