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. 2021 Feb 8;49(4):2375–2389. doi: 10.1093/nar/gkab061

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Grauzone ZAD forms heterodimers with its paralog, CG15073 ZAD. (A) Sequence alignment of Grauzone and CG15073 ZAD sequences (identical residues are shown in red; conserved are in yellow). (B) Crystal structure of Grauzone dimer (PDB ID: 1PZW) colored by homology with CG15073 ZAD according to the sequence alignment shown in panel A. (C) Testing of heterodimerization ability of CG15073 and Grauzone ZADs upon co-expression followed by 6xHis-pulldown assay. Designations are as in the Figure 2D.