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. 2021 Feb 8;49(4):2375–2389. doi: 10.1093/nar/gkab061

Figure 6.

Figure 6.

Chimeric zinc finger-associated domain (ZAD) Zw5 with α2-helix from CG2712 can dimerize with both CG2712 and Zw5 ZADs. (A) Schematic representation of protein chimeras Zw5–2712c (Zw5 ZAD with α2-helix from CG2712 ZAD) and 2712-Zw5c (CG2712 ZAD with α2-helix from ZAD of Zw5). (B) Results of testing of the homo- and heterodimerization abilities of chimeric ZAD Zw5–2712c using a yeast two-hybrid assay. The 2712-Zw5c ZAD demonstrated strong self-activation properties only when fused to Gal4 DNA-binding domain. Designations are as in Figure 4C. (C) Testing of homodimerization ability of chimeras in a chemical crosslinking assay. (D) Results of testing of the homo- and heterodimerization abilities of chimeric ZADs Zw5–2712c and 2712–Zw5c using co-expression in bacteria followed by MBP-pulldown assay. Designations are as in the Figure 2D.