Table 2.

Summary of the impact of amino acid substitutions on homo- and heterodimerization

Substitution	Description	Effect on homodimerization (cross-linking assay)	Effect on heterodimerization (Y2H)	Effect on heterodimerization (MBP pulldown)
CG2712 V16T S82M H84I	H-bond removal (H84I), alter homodimeric hydrophobic interface (V16T)	Weak	Weak	No
CG2712 E58S	Most stable salt bridge removal	Strong	No	Moderate
CG2712 L75Q S76A	H-bond removal (S76A). Alter homodimeric hydrophobic interface (L75Q)	No	No	No
CG2712 M86S	Alter homodimeric hydrophobic interface	Weak	Moderate	Moderate
Zw5 D57E Q75L A76S	engineered salt bridge (D57E)	Weak	No	No
Zw5 M82S	Decrease homodimeric hydrophobic interaction	No	No	No

For details see Supplementary Figure S6.