Figure 11.

Change in cell shape and cell number after direct plasma treatment. (a) RPE-hTERT and (b) CAL27 cells were exposed in PBS to plasma treatment and further incubated (with incubation—early effects) or not (no incubation—immediate effects) in PAP. Photographs were taken immediately (T0 h), 6 h (T6 h) or 24 h (T24 h) after plasma treatment, performed at a gas flow rate of 1 slm (99.8% He/0.2% O2), a treatment distance of 8 mm and a treatment time of 12 min. Photographs were recorded on a Celena^® S digital imaging system (Logos biosystem, Villeneuve d’Ascq, France) at 4$\times$ magnification. Photographs are representative of two independent experiments.