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. 2021 Feb 4;13(4):615. doi: 10.3390/cancers13040615

Figure 12.

Figure 12

The incubation time (early effects) following plasma treatment (immediate effects) is required to achieve efficient cell killing after direct plasma treatment. RPE-hTERT and CAL27 cells were exposed to plasma treatment for 12 min and PAP was either left on the cells for 1 h (Direct) or immediately removed (Plasma, immediate effects). Alternatively, cells were exposed to plasma-activated PBS for 1 h (PAP), or to plasma treatment for 12 min followed by incubation in untreated PBS (Plasma + PBS) or in another PAP (Plasma + PAP) for 1 h. Metabolic cell activity was quantified using a CellTiter-Glo® luminescent assay 24 h post-treatment. Plasma treatments were performed at a gas flow rate of 1 slm (99.8% He/0.2% O2) and a treatment distance of 8 mm. The data are the mean ± SD of 3 to 17 independent experiments. Statistical significance NS: p > 0.05; *: p ≤ 0.05; **: p ≤ 0.01; (t-test).