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. 2021 Feb 4;22(4):1575. doi: 10.3390/ijms22041575

Figure 5.

Figure 5

Cytocompatibility characteristics of the synthesized blocking polypeptide. (A) The ability for AF38Pep to attenuate transforming growth factor-β1 (TGF-β1) induced upregulation in expression of the myofibroblast marker, α-smooth muscle actin (α-SMA), in mouse NIH/3T3 dermal fibroblast cells over 72 h. (B) Cytotoxicity/proliferation assays in TGF-β1 stimulated mouse NIH/3T3 dermal fibroblast cells over 72 h. (C) The ability for AF38Pep to attenuate TGF-β1 induced upregulation in expression of α-SMA in human HFL1 lung fibroblast cells over 72 h. (D) Cytotoxicity/proliferation assays in TGF-β1 stimulated human HFL1 lung fibroblast cells over 72 h. IST-9 antibody was used as a positive control for EDA-FN blockade. Data are representative of three independent experiments and displayed as the mean ± S.E. Statistical analysis is shown as *** p ≤ 0.001, ** p ≤ 0.01, * p ≤ 0.05, and ns = no statistical significance (p > 0.05).