Figure 9.

Assessment of blocking peptide effect on myofibroblast phenotype acquisition. NIH/3T3 fibroblasts were incubated in the presence or absence of 10 ng/mL TGF-β1 with or without 10 µg/mL of peptide for 48 h; immunocytochemistry was used to visualize the marker of myofibroblast differentiation (α-SMA, green; DAPI, blue; top row; original magnification, x200; scale bar, 25 µm) and an indicator of fibroblast activation to a proliferative, migratory and synthetic phenotype (F-actin, green; DAPI, blue; bottom row; original magnification, x200; scale bar, 25 µm). Images are representative of three independent experiments.