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. 2021 Feb 4;13(4):618. doi: 10.3390/cancers13040618

Figure 6.

Figure 6

miR-224-5p involved in the regulation of PD-L1 protein in RCC cells by inhibiting cyclin D1 expression. (A,B) PD-L1 protein abundances in RCC cells were detected by Western blot after transfection of miRNA mimics/inhibitors. The relative density of PD-L1 protein is shown as mean ± s.e.m of triplicate experiments. * p < 0.05; ** p < 0.01; *** p < 0.001. (C) PD-L1 mRNA levels in RCC cells were determined by RT-qPCR after transfection of miRNA mimics/inhibitors (100 nM). Data are mean ± s.e.m. ns, no significant difference. (D) PD-L1 protein abundances in RCC cells were detected by Western blot after treatment of selective CDK4/6 inhibitor palbociclib (1 or 10 μM) for 48 h. (Original Western blots see Figure S7).