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. 2021 Feb 4;13(4):618. doi: 10.3390/cancers13040618

Figure 7.

Figure 7

Regulation of PD-L1 protein expression by miR-224-5p inhibiting cyclin D1 expression could be transmitted between RCC cells by EVs. (A) miR-224-5p levels in RCC-NC/miR-224-5p cells and EVs were determined by RT-qPCR (** p < 0.01; *** p < 0.001). (B) Protein levels of cyclin D1 and PD-L1 in CCND1 transiently expressed 786-O-NC/miR-224-5p cells were detected by Western blot. (C) Protein levels of PD-L1 and cyclin D1 in RCC cells co-culturing with EVs derived from 786-O-NC/miR-224-5p cells for 24 h were detected by Western blot. (D) PD-L1 protein stability in RCC-NC/miR-224-5p cells was detected after CHX (25 mg/mL) treatment for indicated time (h) as shown in the figure. (Original Western blots see Figure S7).