Figure 5.

BALB/c peritoneal macrophages infected with Trypanosoma cruzi and treated with Syzygium aromaticum essential oil or eugenol for 24 hours. Parameters of infection (a-d) and light microscopy (e). Intracellular amastigotes (black arrows) and remains of amastigotes (red arrows) inside macrophages. The images and data (mean ± standard deviation) represent two independent experiments performed in quadruplicate. ^∗p < 0.05 and ^∗∗p < 0.01 when compared with the untreated control group by the Mann–Whitney test. Giemsa, 40x objective.