Figure 6.

The depolarization and Ca²⁺ signaling activated in endothelial cells by the Panx-1 channel blockade depend on the integrity of cholesterol-rich microdomains. (a) Representative recordings of the changes in endothelial cell membrane potential evoked by 60 μM ¹⁰Panx in control conditions and after disrupting the cholesterol-rich signaling microdomains by the treatment with 5 mM methyl-β-cyclodextrin (MβCD) for 30 min. (b) Analysis of the maximum depolarization evoked by ¹⁰Panx in control conditions and after the treatment with MβCD. (c) Time course of the changes in [Ca²⁺]_i observed in primary cultures of endothelial cells in response to ¹⁰Panx application before (control) and after the treatment with MβCD. Values are means ± SEM. ^∗P < 0.05 vs. the vehicle by one-way ANOVA plus the Newman-Keuls post hoc test. ^†P < 0.05 vs. the control by two-way ANOVA.