Fig. 2.
High-throughput screening of a library of natural products against PLpro identifies 5 hit inhibitors. a Results from screening of 1920 natural products for inhibition of PLpro activity. The relative fluorescence units (RFUs) at 10 min after reaction initiation were normalized to DMSO control and used to indicate the enzymatic activities. The dashed line indicates the threshold for hit selection (> 50% fluorescence reduction). b The original reaction progression curves in presence of DMSO or hit compounds. c The structures of the hit compounds