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. Author manuscript; available in PMC: 2022 Mar 1.
Published in final edited form as: Arthritis Rheumatol. 2021 Feb 8;73(3):520–529. doi: 10.1002/art.41536

Figure 2. Effect of iloprost on EC angiogenesis.

Figure 2.

Angiogenesis of ECs was measured using an in vitro Matrigel tube formation assay. For A and C, ECs were pre-treated with iloprost (150nM) overnight before they were plated on Matrigel. After 6 hours, cells were fixed and stained. For B, BV9 (25 μg/ml) was used to pre-treat the ECs for 30 min before iloprost was added. The cells were cultured for 8 hours before they were fixed and stained. (A) and (B) Iloprost increased tubulogenesis in normal ECs and this was reduced by a function blocking antibody to VE-cadherin (BV9); (C) Iloprost induced tubulogenesis in SSc ECs. Experiments were done with 3 subject-derived lines. Results are expressed as mean +/− SD and p<0.05 was considered significant.