Fig. 5.

Electrophysiological properties of human iPSC-derived 5-HT specific neurons. a Voltage-gated Na + and K + currents triggered by voltage steps from -100 to + 70 mV at a holding potential of − 80 mV. b Action potentials were induced upon injection of current steps from − 120 to + 120 pA. Depicted are traces at -100 pA and at 0 pA with spontaneous action potentials. c Single action potential on an expanded time scale. Indicated are amplitude, HHW and afterhyperpolarization (AHP). d Neurons display two typical modes of activity: bursts with intercalated pauses (upper trace) or activity with constant frequency (lower trace). e–h Bar graphs of electrophysiological properties of human iPSC-derived 5-HT specific neurons over a time period of 6 weeks. e AP amplitude, f half-height width (HHW), g resting membrane potential and h AP frequency. Electrophysiological signature characteristic of serotonergic neuron was verified using the JMUi001-A iPS line (data not shown). Pairwise post-hoc t-tests, * P < .05, ** P < .01, *** P < .001