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. Author manuscript; available in PMC: 2022 Mar 1.
Published in final edited form as: FEBS J. 2020 Sep 25;288(6):1871–1886. doi: 10.1111/febs.15541

Figure 4. Blockade of C5a, CCL2 or CXCL1 attenuate PIN cell proliferation induced by macrophage conditioned media.

Figure 4.

(A-D) Pr111 cells grown on matrigel in 3D were treated with Raw264.7 macrophage conditioned media in the presence of isotype control antibody or the neutralizing antibody of C5a (A), CXCL1 (B), CCL2 (C) or all three (D) for 72 h. The cells were fixed, permeabilized and immunostained with cyclin D1 (green). The cell nuclei were stained and visualized with DAPI (blue). Cell proliferation index of Pr111 cells under these conditions was quantified. Scale bar: 25 μm. The data was analyzed by student t test. *: p<0.05 as compared to isotype control antibody, n=3.