Figure 10.

(A) In vivo SPECT-CT images of ^99mTc-labelled EMVs derived from mouse macrophages and human neural stem cells, compared with ^99mTc-labelled sEVs derived from mouse macrophages and free [^99mTc]Tc-HMPAO 3 h post injection. Figures taken with permission from Hwang et al. ⁸⁷ (B) Schematic representation of the protocol for radiolabelling red blood cell-derived EMVs with ^99mTc. (C) In vivo gamma camera images of iv. administered free ^99mTc and ^99mTc-EMVs in male C57BL/6 mice 1 h and 3 h post injection. (D) Quantification of free ^99mTc and ^99mTc-EMVs injected in organs of interest 1 h and 3 h post injection; data presented as mean ± SD of n = 4 and analysed by Student's t-test. Figures adapted with permission from Gangadaran et al. ⁸⁸.