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. 2021 Jan 30;11(8):3781–3795. doi: 10.7150/thno.49288

Figure 2.

Figure 2

The absence of granzyme A increases survival in mice during CLP and E. coli-induced sepsis. Sepsis was induced by CLP in WT and GZMA-/- mice (A) and GZMA-/- and GZMA+/+ littermates (B) as described in materials and methods. After 6 h, a mixture of antibiotics, ceftriaxone (30 mg/kg) + Metronidazole (12.5 mg/kg) was administered i.p. every 24 h for 5 days. WT and GZMA-/- sham operated mice underwent the same procedure but without the ligation and puncture of the cecum. Survival was monitored during 14 days. The data correspond to the indicated number of biological replicates (individual mice) from three independent experiments. Statistical analyse was performed using logrank and Gehan-Wilcoxon test. *p < 0.05. (C) WT and GZMA-/- mice were infected i.p. with 2 x 108 CFU of E. coli as described in materials and methods. The data corresponds to the indicated number of biological replicates from two independent experiments. Statistical analyse was performed using logrank and Gehan-Wilcoxon test. *p < 0.05. (D) An in vitro analysis was performed to determine the capacity of GzmA in the control of bacterial pathogens. M1 macrophages were differentiated as described in material and methods. Macrophages were incubated with GZMA (300 nM), infected with E. coli (MOI 1:100) and incubated for 4, 24 and 48 h at 37 °C. After incubation time cells were lysed and the number of CFU was determined. Data are presented as mean ± SEM from 3 independent experiments.