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. 2021 Jan 30;11(8):3781–3795. doi: 10.7150/thno.49288

Figure 3.

Figure 3

The absence of granzyme A reduces the level of proinflammatory cytokines during sepsis induced by CLP. Sepsis was induced by CLP in WT and GZMA-/- mice as described in materials and methods. After 6 h, a mixture of antibiotics, ceftriaxone (30 mg/kg) + metronidazole (12.5 mg/kg) was administered every 12 h. WT and GZMA-/- sham operated mice underwent the same procedure but without CLP. After 6 or 24 h of sepsis induction, mice were sacrificed and the levels of IL-1α, IL-1β, TNFα and IL-6 in plasma and peritoneal lavage fluids were determined by ELISA. Data are presented as mean ± SEM of 5 (sham) or at least 8 (CLP) different biological replicates (individual mice) from 3 independent experiments. Statistical analysis was performed by one-way ANOVA test with Bonferroni's post-test *P < 0.05; **P < 0.01; ***P < 0.001.