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. 2021 Jan 27;11(8):3742–3759. doi: 10.7150/thno.53023

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PRMT5 associates with EZH2 in CRC cells. (A) Co-immunoprecipitation of endogenous EZH2 from HCT116 and SW480 cells overexpressing Flag-tagged PRMT5. IgG was used as the negative control. (B) Western blot analysis of EZH2 binding to purified GST and GST-tagged PRMT5 using EZH2 antibody (top). GST and GST-tagged PRMT5 from E. coli BL21 (DE3) strain were visualized by staining with Coomassie brilliant blue R-250 (bottom). The red asterisk and black asterisk were GST and GST-tagged PRMT5, respectively. (C) The subcellular location of endogenous PRMT5 and EZH2 proteins was analyzed in HCT116 and SW480 cells by immunofluorescence microscopy. Scale bar: 10 µm.