PRMT5 and EZH2 are elevated and negatively correlated with CDKN2B in CRC samples. (A) Representative images of IHC staining of PRMT5, EZH2 and CDKN2B in 80 cases of CRC patients and their adjacent normal tissues. Scale bar: 200 µm. (B) H score of PRMT5 (left), EZH2 (middle) and CDKN2B (right) in CRC (n = 80) and adjacent normal (n = 80) tissues, **P < 0.01. (C) Overall survival of patients with CRC was performed by Kaplan-Meier analysis. High and low PRMT5 (left), high and low EZH2 (middle) and high and low CDKN2B (right). P < 0.01. (D) Correlations of CDKN2B and PRMT5 (left; r2 = 0.3411, P < 0.01), CDKN2B and EZH2 (middle; r2 = 0.2113, P < 0.01) and EZH2 and PRMT5 (right; r2 = 0.3423, P < 0.01) protein levels in CRC tissues (n =80) were performed by Spearman's correlation analysis.