Figure 2.

Osimertinib decreases DR4 levels in an EGFRm NSCLC xenografts in vivo accompanied with induction of apoptosis. PC-9 xenografts at ~ 300 mm³ were treated with vehicle control (Ctrl) or osimertinib (Osim; 10 mg/kg; og; once/daily) and collected at the indicated times (N = 3). The given proteins were detected with Western blotting (A). The results or band intensities were quantified with NIH Image J software (B and D) in comparison with tumor weight alteration (C). The data presented in B-D are means ± SEs (N = 3). Control and tumor tissues on day 9 were also stained for cPARP with IHC (E). The scale bar is 50 μm. CF, cleaved form.