Skip to main content
. 2021 Feb 15;11(8):3964–3980. doi: 10.7150/thno.54824

Figure 7.

Figure 7

DR4 knockdown in osimertinib-resistant cell lines sensitizes the cells to osimertinib-induced apoptosis, whereas MEK inhibition combined with osimertinib potentiates DR4 reduction with augmented induction of apoptosis in osimertinib-resistant cells. A-C, The indicated osimertinib-resistant cell lines were infected with DR4 shRNA lentiviruses for 24 h followed by treatment with 100 nM osimertinib (Osim) for another 48 h. DR4 knockdown and PARP cleavage were detected by Western blotting and apoptosis was measured by annexin V/flow cytometry. D and E, PC-9/AR cells were treated with 100 nM osimertinib alone, 25 nM trametinib (Tram) or 50 nM selumetinib (Sel) or PD0325901 (PD901) alone, and the combination of osimertinib with a given MEK inhibitor for 24 h (D) or 8 h (E). The cells were then harvested for Western blotting to detect the given proteins (D and E) and for annexin V/flow cytometric analysis to detect apoptosis (D). **, P < 0.01; *** P < 0.001.CF, cleaved form.