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. 2021 Feb 5;10(2):340. doi: 10.3390/cells10020340

Figure 1.

Figure 1

Cannabinoids reduce viability of GB and GSC cells. CBG (A,D), CBD (B,E), and THC (C,F) impaired the viability of differentiated GB cells and GSCs. Dose responses of cell viability measured by MTT assay (y-axis) and different CBG, CBD and THC concentrations increasing in the range of 0.1–50 µM (x-axis log scale) on established GB cells (U87 and U373) (A–C; red lines) in comparison to primary patient-derived GB cells (A–C; black lines). Established GSC lines NCH421k and NCH644 (D–F; red lines) are compared to primary patient-derived GSCs (D–F; black line) after 48 h of a single treatment with cannabinoids. Data are expressed as mean ± SE (n = 3–5 independent biological experiments, each in technical triplicate). Vehicles comprised ≤ 0.1% (v/v) DMSO for THC and CBD and 0.24% (v/v) ethanol for CBG. The methodology taken from Deng et al. [41] is described in great detail in Material and Methods (Section 2.5).