Skip to main content
. 2021 Feb 6;22(4):1654. doi: 10.3390/ijms22041654

Figure 6.

Figure 6

Effect on AHR-HSC70 interaction in the presence or absence of Q18 or 6-AN in MDA-MB-468 cells. Cells were treated with (A) 10 μM of Q18 for 0, 4, or 8 h or (B) DMSO or 10 μM of 6-AN for 24 h, followed by the immunoprecipitation of 2 mg of whole cell lysates with AHR antibody SA-210 (IP: AHR). The Western bands were detected using protein-specific antibodies (SA-210 for AHR, B-6 for HSC70, and H4B4 for LAMP2A). Input represents 1% of sample to start the experiment. One control (0 h for A and DMSO for B) of each data set was arbitrarily set as one to normalize the three independent experimental data sets, thus no error bar was presented for these conditions. Results are means ± SD of three independent experiments. Two-tailed unpaired t test with Welch’s correction were used for statistical analysis. * p < 0.05 when compared to the control. ns, not significant.