Skip to main content
. 2021 Feb 6;22(4):1654. doi: 10.3390/ijms22041654

Figure 8.

Figure 8

Q18 effect on the cyp1a1 transcript and AHR protein levels in MDA-MB-468 cells. (A) Effect on the cyp1a1 transcript levels when MDA-MB-468 cells were treated with Q18. At each timepoint, cells were treated with either 10 μM of Q18 or with vehicle (DMSO). RT-qPCR was performed as described in the Materials and Methods section. The fold changes of gene transcript levels between Q18 treated and DMSO vehicle control treated cells were normalized by 18S. One-way ANOVA with Dunnett’s multiple comparisons test was used in analyzing the Q18 treatment data. **** p < 0.0001 showed that there is a significant difference in the cyp1a1 mRNA levels at the 24 h timepoint versus zero timepoint. The error bars represent means ± SD, n = 3. (B) Western blot analysis showing the effect of CH223191 on the AHR protein levels in MDA-MB-468 cells. Cells were treated with 10 μM of CH223191, 10 μM of Q18, or DMSO (vehicle) for 24 h. All lanes contain 60 μg of whole cell lysates. The amount of AHR was normalized by total protein stain. The normalized value of AHR from the treatment group was divided by the normalized value from DMSO treatment to calculate the relative abundance of AHR. The error bar represents mean ± SD of three independent experiments.