Figure 1.

Experimental design and paradigm. Organotypic hippocampal slice cultures (OHSCs) were cultured for 3 w and 9 w. Slices were treated with propidium iodide (PI) 2 h before 5 µM kinetic acid (KA) treatment for all cultures (PRE). All received KA treatment except for the normal controls in each experimental group. After 18 h exposure to KA, the medium containing KA was changed to a fresh culture medium containing 500 µM ascorbic acid (AA). PI uptake, Western blot, and electrophysiological studies were conducted 24 h after AA treatment. Experimental groups were divided by culture time and treatment type, such as 3 w, 9 w, and 9 w-daily. In the 9 w-daily group, 500 µM AA was added to the culture medium for 6 w before studies began.