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. 2021 Feb 6;10(2):344. doi: 10.3390/cells10020344

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Cisplatin (at 10 μM) promoted the tumoroid growth of mCRC cells: (A) a scheme of the experimental protocol used for panel B–G. LuM1/m9 cells were pre-cultured for 4 days in mTeSR1 on NanoCulture Plate^® (NCP), and then Cisplatin at 10 μM was added; (B) representative images of tumoroids. Scale bars, 500 μm; (C) column scatters the plotting of tumoroid size. Representative data from a single well were shown; (D) average tumoroid size. n = 2 or 3 independent culture wells; (E) the number of tumoroids larger than 300 μm². n = 2 or 3 independent culture wells; (F) Mmp9 promoter activity. n = 3 independent culture wells; and (G) cell viability. n = 3 independent culture wells. * p < 0.05, n.s., not significant.