Figure 12.

Real-time RT–PCR analysis of miRNA target gene expression in HCT116 cells treated with butyrate for 24 h. mRNA levels of (A) EIF4G2 and miR-542 predicted target gene (B) BIRC5 in HCT116 cells transfected with miRNA or NC mimics for 48 h, followed by 24 h of treatment with 0 mM or 2.5 mM butyrate, over a 72 h post-transfection period. The mean mRNA levels ± SEM (n = 3) are represented, and their expression is normalized to the geometric mean of three reference genes, ACTB, B2M and GAPDH. Significant values are indicated by ** p < 0.01, *** p < 0.001, **** p < 0.0001. NC = negative control mimic.