Table 1.
Methods to screen TFEB agonists.
| Method | Function | Detection Indicator | Reference |
|---|---|---|---|
| Nuclear translocation | When TFEB is activated, it is displaced into the nucleus, so it is possible to determine whether TFEB is activated by observing the nucleus translocation. | DAPI, GFP, FITC, EB | Najibi, et al., 2016 [71] |
| Gene expression (qPCR) | Fluorescence chemicals were used to measure the total amount of products after each PCR cycle | CT value | Bao, et al., 2016 [72] |
| Promoter activation | The initiation of transcription is the key stage of gene expression, and promoter activation increases the level of gene expression. | Target protein | Ghosh, et al., 2015 [53] |
| Autophagy flux | Observing the accumulation of autophagosomes can reflect the induction of autophagosomes and the reduction of autophagosome consumption | mRFP-GFP-LC3, LC3-I, LC3-II | Castillo, et al., 2013 [73] Wang, et al., 2009 [74] |
| Protein expression (WB) | TFEB is activated and the amount of target protein produced increases. | Target protein | Dai, et al., 2020 [75] |
| Electrophysiology | Membrane potential changes as substances cross cell membranes, and TFEB changes are observed by recording the electrical activity of cells in the body. | Transmembrane current, Action potential | Kosacka, et al., 2013 [76] |
| A UPS-enabled high-throughput screen | Trace, fast, sensitive, and accurate screening the TFEB agonists. | PH, LAMP1 | Wang, et al., 2017 [77] |