Table 1.

Enzymatic assays on HsPim-1 and HsPim-2 (ADP-Glo method at 10 μM ATP).

Entry	Compound	X	HsPim-1 IC50 (µM) 1	HsPim-2 IC50 (µM) 1
1	1	H	0.074	2.10
2	5a	6-F	0.17	0.76
3	5b	7-F	1.24	4.20
4	5c	6-Cl	0.13	0.17
5	5d	7-Cl	2.10	2.40
6	5e	6-Br	0.16	0.58
7	5f	7-Br	0.18	2.20
8	5g	6-CF3	0.20	1.80
9	5h	7-CF3	3.85	6.40
10	SGI-1776		0.05	0.10

¹ IC₅₀ on Pim-1/2 kinase activity were calculated from dose−response curves. Each inhibitor concentration was tested in duplicate. Inhibition curves for 5c and 5e are reported in supplementary data. Hs: Homo sapiens. Values are a mean of n ≥ 3 independent experiments. Hs: Homo sapiens.