Effects of eugenol on the viability of NCM460, Caco2 and SW620 cell lines. Effects of eugenol on the viability of NCM460, Caco2 and SW620 cell lines at three timepoints: 24, 48, 72 h. CTRL: control untreated cells; NCM460: human normal colon mucosal epithelial cells; Caco2: human epithelial colorectal adenocarcinoma cells; SW620: colon cancer cells derived from lymph node metastatic site. The values were normalized to the untreated controls. The results are expressed as average ± SE of three independent experiments. Significant statistical differences were evaluated with one-way ANOVA, followed by Bonferroni’s Multiple Comparison test: * p < 0.05; ** p < 0.01; *** p < 0.001.