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Apoptosis detection. Apoptosis detection by AnnexinV/PI analysis on NCM460 (A–C), Caco2 (D–F), SW620 (G–I). Ctr: control untreated cells. Cells were exposed to treatments with 800 µM eugenol (E800) and 75 µM cinnamaldehyde (A75) for 72 h. Viable cells (lower left, AnnexinV −/PI −), early apoptotic (lower right, AnnexinV +/PI −) and late apoptotic/necrotic cells (upper, AnnexinV +/PI +) quadrant. The results shown are representative of three independent experiments.
