Figure 2.

Epididymal white adipose tissue (eWAT) weights and expression of adipogenic genes. (A) Epididymal white adipose tissue was dissected and its weight measured after 10 weeks of dietary intervention. The expression of adipogenic genes, (B) ppar-γ and (C) srebp-1c, was assessed by qRT-PCR. Relative transcription levels of the target gene were calculated by normalization to β-actin using the delta–delta Ct method (2^−ΔΔCt). Data are expressed as means ± SEM. Different letters indicate significant differences between groups (p < 0.05) (ND, normal diet; HFD, high fat diet; HFD-RBO, high-fat diet-rice bran oil; HFD-PO, high-fat diet-palm oil).