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. 2021 Feb 7;10(2):359. doi: 10.3390/foods10020359

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Expression of macrophage polarization markers in BMDMs. The BMDMs were stained with specific antibodies and mean fluorescence intensity (MFI) was assessed by flow cytometry. The F4/80⁺CD11b⁺ population cells were gated and designated as macrophages. The expression of (A,C,E) CD11c as an M1 marker and (B,D,F) CD206 as an M2 marker was further assessed in non-polarized (M0), polarized M1 BMDMs, and M2 BMDMs. Results were normalized to the MFI of the ND-fed control. n.s. no significance. Values are expressed as means ± SEM, and different letters indicate statistical significance (p < 0.05) (ND, normal diet; HFD, high fat diet; HFD-RBO, high-fat diet-rice bran oil; HFD-PO, high-fat diet-palm oil).