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. 2021 Feb 9;11(2):449. doi: 10.3390/ani11020449

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Immunofluorescence of NPY (green) and 5-HT (red) in the abomasum, duodenum and pancreas of goat kids fed with red orange and lemon extract (RLE) supplementation. NPY distributed in sparse cells of abomasal epithelium (a1) and 5-HT in glands at the basis of gastric epithelium (a2). Absence of NPY/5-HT co-localization (a1+a2). NPY (b1) and 5-HT (b2) co-distributed in the neuroendocrine cells of duodenum, with NPY mainly localized in the cytoplasm and 5-HT on the cellular membrane (b1+b2). NPY (c1) and 5-HT (c2) in the pancreatic islet, localized in different cells (c1+c2). Scale bar: (a) = 25 µm; (b,c) = 50 µm.