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. 2021 Feb 5;22(4):1616. doi: 10.3390/ijms22041616

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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TGF-β1 treatment (closed triangles, OD_540nm: 0.27 ± 0.027/well) of CoReFs for 8 days increased collagen deposition compared to the control (open triangles, OD_540nm: 0.19 ± 0.015/well), as measured by Sirius red staining. An OD_540nm value of 0.20 corresponds to 20 µg type I collagen. Mean ± SEM (n = 12).