Figure 5.

CoReFs treated without (A–C) or with 1 ng/mL TGF-β1 for 8 days (D–F), fixed and stained with Sirius red (B,E) or crystal violet (C,F). OD_540nm for (B) was 0.22, and for (E) was 0.59. OD_590nm for (C) was 0.54 and for (F) was 1.46. Scale bars, 100 µm. Cells in (A) and (D) were trypsinized (2 mL 0.05% trypsin-0.02% EDTA/well for 10 min at 37 °C) and counted by a Countess™ automated cell counter (Thermo Fisher Scientific, Waltham, MA, USA). The number of cells in (A) was 0.77 × 10⁶ and in (D) was 1.2 × 10⁶.