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. 2021 Feb 5;22(4):1616. doi: 10.3390/ijms22041616

Figure 6.

Figure 6

Effect of TGF-β1 (1 ng/mL) treatment of CoReFs for 48 h on COL1A1, COL3A1, COL6A1, MMP2, and ACTA2 mRNA levels (A) and cell proliferation measured by WST-1/BrdU assays (B). Phase contrast photomicrographs (Olympus CK40 with Moticam® S2) of CoReFs treated without (C) or with TGF-β1 (D) for 48 h just before adding the WST-1 reagent. Scale bars, 100 µm. (A) mRNA levels were normalized to GAPDH, expressed as fold change relative to control-treated CoReFs and log-transformed. Geometric mean ± back-transformed SEM of 6 replicates is shown. (B) Mean ± SEM of 10 replicates is shown. ** p < 0.01, *** p < 0.001 vs. control.