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. 2021 Feb 5;22(4):1616. doi: 10.3390/ijms22041616

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of TGF-β1 on collagen deposition (A), cell numbers (B), and MMP-2 (C) of CoReFs treated for 8 days at three different concentrations (0 [control], 0.01, 0.1 and 1 ng/mL). (A,B) Mean ± SEM (n = 6). * p < 0.05, *** p < 0.001 vs. control. (C) A representative zymogram (inverse image) of MMP-2 in the conditioned media (5 µL/lane) from CoReFs (3 patients). The estimated MMP-2 content (pg) is indicated below each lane of the zymogram from the densitometric determination of band intensities by ImageJ.