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. 2021 Feb 8;22(4):1703. doi: 10.3390/ijms22041703

Figure 4.

Figure 4

Stationary phase-specific transfer RNA fragments. (a) Northern blot analysis probing for 3′-tRFLeuU (FM_-NC_000913.2_3320093_38), 5′-tRFGly (FM_+NC_000913.2_4390382_32), and 5′-tRFAlaV (FM_+NC_000913.2_0225499_28). 5S rRNA was used as a loading control. Zero point four and 2 were optical density measurements, h: hours, d: days. tRF = tRNA derived RNA fragment. (b) Northern blot analysis probing for 3′-tRFLeuU, 5′-tRFGly, and 5′-tRFAlaV. P100 = pellet enriched for ribosome-associated RNA. S100 = supernatant containing non-ribosome associated RNA.