Table 1.
E2-regulated proteins in OVX rat uterus verified by targeted proteomics and applied to testing BPA [34,53,54,55,56,57,58] as a well-known estrogenic EDC.
| Rat Uterus Protein | Proteotypic Tryptic Peptide | Fold Change after E2 Treatment 1 | Fold Change after BPA Treatment 1 |
|---|---|---|---|
| Transglutaminase 2, C polypeptide (TGM2) | YSGCLTESNLIK | 24.7 ± 4.6 | 14.3 ± 4.4 |
| SEGTYCCGPVSVR | 17.5 ± 2.3 | 10.2 ± 2.1 | |
| Elongation factor 2 (EEF2) | EGIPALDNFLDKL | 3.9 ± 0.3 | 3.2 ± 0.3 |
| TFCQLILDPIFK | 8.4 ± 1.1 | 6.5 ± 1.1 | |
| Selenium-binding protein 1 (SELENBP1) | HEIIQTLQMK | –2.2 ± 0.5 | –2.0 ± 0.2 |
| LILPSIISSR | –2.9 ± 0.1 | –2.3 ± 0.2 | |
| Lumican (LUM) | NNQIDHIDEK | –3.8 ± 0.8 | –6.3 ± 0.8 |
| SLEYLDLSFNQMSK | –2.0 ± 0.4 | –2.5 ± 0.1 |
1 All reported results (data given as average ± standard error) yielded statistically significant differences from OVX controls with analysis of variance (ANOVA) followed by post hoc Dunnett’s tests, p < 0.05.