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Percentages of viable spermatozoa with an intact acrosome in extended and frozen-thawed (FT) samples at 30 min and 240 min post-thaw. In experiment 1 (n = 8), TEA, PAX, and 2-GBI blockers were added to LEY medium at 15 °C (a), whereas in experiment 2 (n = 9), they were added to LEYGO medium at 5 °C (b). Different superscripts indicate significant differences (P < 0.05) between samples within the same time point. Results are given as mean ± SEM.
